The study aimed to isolate and diagnose Pseudomonas aerginosae from clinical specimens of diabetic foot infection patients, study the resistance of bacteria to antimicrobial agent and molecular detection of class 1, 2 and 3 integron-associated gene cassettes from Pseudomonas aeruginosa in Najaf province. Sensitivity test was examined via disk spread, method antibiotic, the insulated shows 100% resistance to cefepim, amoxiclave, ceftazidime, gentamicin, cefotaxime and cephalexine though the carry-on of the antibiotics showed various activity against the isolates. At molecular study, the investigated the presence of integrin's genes (intI, intII and intIII) using PCR technique and electrophoresis systems, PCR analysis showed that the integrin IntI, IntII and IntIII were detected in 12/30, 16/30 and 6/30 respectively in Pseudomonas aeruginosa isolates.