The present study included (50) clinical samples were collected from patients suffering from tonsillitis signs during the period from) November 2018 to January 2019). All specimens were cultured for macroscopic and microscopic study. Results show that out of 50 patients, the male was 60%, and 40% were female. Several morphological, physiological, and biochemical tests showed that P. aeruginosa constituted 18 isolates (36%) of these isolates. P. aeruginosa isolates were 18 isolates diagnosed by the morphological, cultural, and biochemical characters; the identification was confirmed by automated VITEK-2 compact system and molecular method for the presence of OprL. The results showed that only 12 (66.6%) isolates diagnosed as P. aeruginosa by automated VITEK-2 compact system, and that was carrying OprL, which are diagnosed as P. aeruginosa by PCR. According to the different diagnostic above, VITEK and PCR method were more sensitivity for P. aeruginosa detection among tonsillitis patients
The present study included (50) clinical samples were collected from patients suffering from tonsillitis signs during the period from) November 2018 to January 2019). All specimens were cultured for macroscopic and microscopic study. Results show that out of 50 patients, the male was 60%, and 40% were female. Several morphological, physiological, and biochemical tests showed that P. aeruginosa constituted 18 isolates (36%) of these isolates. P. aeruginosa isolates were 18 isolates diagnosed by the morphological, cultural, and biochemical characters; the identification was confirmed by automated VITEK-2 compact system and molecular method for the presence of OprL. The results showed that only 12 (66.6%) isolates diagnosed as P. aeruginosa by automated VITEK-2 compact system, and that was carrying OprL, which are diagnosed as P. aeruginosa by PCR. According to the different diagnostic above, VITEK and PCR method were more sensitivity for P. aeruginosa detection among tonsillitis patients