Salmonella typhi the causative agent of typhoid, the consider largest killer disease is becoming a major threat due to emerging antimicrobial resistance. Mechanisms underlying bacterial drug resistance involve developing and/or acquiring of genetic elements in the form of variety of genes, gene complexes or mutations.These genetic elements are either chromosomal and/or extrachromosomal in the form of plasmids. The present study was conducted to investigation of the presence antibiotic-resistance genes (cat, dfrA7, gyrA, gyrB, strA-strB, sulI, sulII, tetB) were done by using PCR technique and electrophoresis systems. One hundred percent of S.typhi isolates were positive with gyrA, while 25 (83%) of isolates carry gyrB gene, 19 (63.3%) of isolates carry gene cat, dfrA7, 12(40%) and 6 (20%) isolates were carry sulII and sulI, respectively. Thirty (100%) of the isolates gave a negative genes results of (strA-strB, tetB).
Salmonella typhi the causative agent of typhoid, the consider largest killer disease is becoming a major threat due to emerging antimicrobial resistance. Mechanisms underlying bacterial drug resistance involve developing and/or acquiring of genetic elements in the form of variety of genes, gene complexes or mutations.These genetic elements are either chromosomal and/or extrachromosomal in the form of plasmids. The present study was conducted to investigation of the presence antibiotic-resistance genes (cat, dfrA7, gyrA, gyrB, strA-strB, sulI, sulII, tetB) were done by using PCR technique and electrophoresis systems. One hundred percent of S.typhi isolates were positive with gyrA, while 25 (83%) of isolates carry gyrB gene, 19 (63.3%) of isolates carry gene cat, dfrA7, 12(40%) and 6 (20%) isolates were carry sulII and sulI, respectively. Thirty (100%) of the isolates gave a negative genes results of (strA-strB, tetB).