Molecular detection of spa gene among Staphylococcus aureus isolated from mastitis Abstract: From June to September 2020, among thirty gram-positive bacteria grown with mannitol salt agar, seventeen Staphylococcus aureus isolates were described. Staphylococcus aureus isolates were described in the initial identification based on the colonial morphology, microscopic analysis, and biochemical tests. The final identification was performed using an automated VITEK-2 compact system. The spa genes were detected by the genotyping method using the polymerase chain reaction (PCR) technique. The results showed that the spa gene comprised 19 isolates (63.3%) of S. aureus. كلمات دلاليه Firmicutes, PCR, Spa gene, Mastitis الكتاب: اسراء عبد الامير فليح link: https://alkafeel.edu.iq/research/details/265